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KMID : 0903519710140030177
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1971 Volume.14 No. 3 p.177 ~ p.182
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Fractionation of Iron in Rice Leaf Tissue
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Abstract
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A new method for the measure of iron pools using 0.02M EDTA and Na₂S₂O₄ was tested on Akagare diseased and healthy rice leaf tissue
1) The proposed method could fraction iron into four fractions; ferrous iron(Fe^(++)), ferric iron(Fe^(+++)) precipitated iron(PFe) and bound iron(BFe) well indicating the physiological status of tissue.
2) The pattern of iron pools appears to be Fe^(+++)$gt;PFe$gt;BFe$gt;Fe^(++) in most physiologically favorable status of iron, PFe$gt;Fe^(+++)$gt;BFe$gt;Fe^(++) in favorable status, BFe$gt;Fe^(+++)$gt;PFe$gt;Fe^(++) in unfavorable status and BFe$gt;PFe$gt;Fe^(+++)$gt;Fe^(++) in toxic status.
3) The percentage of each fraction to total iron was less than 10 for Fe^(++), 20 to 40 for Fe^(+++) and PFe and 20 to 50 for BFe.
4) Ferrous iron was always higher in upper half leaf, the appearance of which is less healthier than lower half indicating that there is more active metabolic system in which ferrous iron is involved.
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